Journal: Gene Regulation and Systems Biology

Article Title: Transcriptional Response Of E. coli Upon FimH-mediated Fimbrial Adhesion

doi:

Figure Lengend Snippet: Differentially expressed genes (≥1.5-fold) in E. coli CSH50 cells attached to mannose agarose beads versus unattached cells.

Article Snippet: The Operon E. coli Genome Oligo Set version 1.0 (Operon Biotechnologies, Inc.) is an anti-sense oligo set consisting of 70-mer probes representing 5,978 ORFs derived from three strains of E. coli along with additional positive and negative controls.

Techniques: Activity Assay, Binding Assay, Membrane

Journal: Gene Regulation and Systems Biology

Article Title: Transcriptional Response Of E. coli Upon FimH-mediated Fimbrial Adhesion

doi:

Figure Lengend Snippet: Heat map generated via hierarchical clustering analysis showing differentially altered genes in a temporal manner during E. coli attachment. These genes were organized using a hierarchical clustering algorithm (Metabominer; Wishartlab) so that those which display similar expression patterns were grouped together. The hierarchical cluster analysis was performed using average agglomeration method. The heatmap was plotted with gene (row) normalized and the distance between genes were calculated based on Euclidean distance. A color bar is represented at the top of the panel with a range from −2 to 2 (blue to red), with red color representating up-regulation while blue color representing down-regulation.

Article Snippet: The Operon E. coli Genome Oligo Set version 1.0 (Operon Biotechnologies, Inc.) is an anti-sense oligo set consisting of 70-mer probes representing 5,978 ORFs derived from three strains of E. coli along with additional positive and negative controls.

Techniques: Generated, Expressing